human pdgf r beta antibody  (Bio-Techne corporation)

Journal: Stem Cell Research & Therapy

Article Title: Single-cell transcriptomics reveals notch regulation in quiescent LEPR⁺ endometrial mesenchymal stem cells

doi: 10.1186/s13287-025-04803-7

Figure Lengend Snippet: The expression and location of leptin receptor (LEPR) in human endometrium and its relationship with endometrial mesenchymal stem cells (eMSCs). A Representative contour plots showing the expression of LEPR in freshly isolated endometrial stromal cells and eMSCs (n = 10). B The expression of LEPR at different menstrual phase of freshly isolated endometrial stromal cells and eMSCs (proliferative: n = 5; secretory: n = 5). C Representative contour plots showing the expression of LEPR in cultured endometrial stromal cells and eMSCs (n = 12). D The expression of LEPR in different menstrual phases of cultured endometrial stromal cells and eMSCs (proliferative: n = 5; secretory: n = 7). Full-thickness human endometrial sections immunohistochemical stained with LEPR from E proliferative and F secretory phase of the menstrual cycle. Isotype controls for G proliferative and H secretory sample, respectively. *, blood vessel; GE, glandular epithelium; S, stroma. (Scale bar: 50 µm). I Representative immunofluorescent images showing the co-localization (white arrows) of CD140b (green; pericytes), CD146 (red; endothelial) and LEPR (pink) in blood vessels in the endometrial stroma. (Scale bar: 10 µm). Results are presented as mean ± SD; **, P < 0 .01

Article Snippet: After reaching 80% confluence in culture, endometrial stromal cells were incubated with Phycoerythrin (PE)-conjugated anti-CD140b antibody (R&D Systems, USA, #FAB1263P) at 4 °C for 45 min, and then with anti-mouse IgG1 magnetic microbeads (Miltenyi Biotech, #130–047-102) at 4 °C for 15 min.

Techniques: Expressing, Isolation, Cell Culture, Immunohistochemical staining, Staining

Journal: Stem Cell Research & Therapy

Article Title: Single-cell transcriptomics reveals notch regulation in quiescent LEPR⁺ endometrial mesenchymal stem cells

doi: 10.1186/s13287-025-04803-7

Figure Lengend Snippet: The role and expression of Notch-related ligands and receptors in different endometrial stromal subsets. A Heatmap representation of the number of potential ligand-receptor pairs among different cell types of human endometrial cells. B The cell–cell interaction network was established by CellPhoneDB. C Ligand-receptor pairs of different cell types determined with CellPhoneDB are displayed with bubble plots. D The gene expression pattern of NOTCH-related molecules in stromal (yellow), eMSC (green), LEPR − eMSC (blue), LEPR + eMSC (red) from a primary human endometrial single-cell sequencing dataset (n = 10). E Quantification of HES1, NOTCH1, NOTCH2, and NOTCH4 in freshly isolated human endometrial stromal subsets (n = 8). Representative immunofluorescent images showing the co-localization (white arrows) of CD146 (green), CD140b (yellow), LEPR (pink), DLL1(orange) and JAG1(red) in blood vessels in the endometrial stroma. S, stroma; BV, blood vessels (Scale bar: 20 µm). The results are presented as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: After reaching 80% confluence in culture, endometrial stromal cells were incubated with Phycoerythrin (PE)-conjugated anti-CD140b antibody (R&D Systems, USA, #FAB1263P) at 4 °C for 45 min, and then with anti-mouse IgG1 magnetic microbeads (Miltenyi Biotech, #130–047-102) at 4 °C for 15 min.

Techniques: Expressing, Gene Expression, Sequencing, Isolation